Determination of proteins in infant formula by high-performance liquid chromatography-electrospray tandem mass spectrometry

J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jan 1;845(1):69-73. doi: 10.1016/j.jchromb.2006.07.036. Epub 2006 Sep 5.

Abstract

To determine the protein content of formula, gel electrophoresis was performed on the infant formula samples and the entire protein patterns were analyzed by nano-high performance liquid chromatography-electrospray tandem mass spectrometry (nano-HPLC/ESI/MS/MS). From the commercial infant formula profiled in this study, a total of 154 peptides, corresponding to 31 unique proteins were identified by nano-HPLC/ESI/MS/MS. Each of the identified peptides was reconfirmed by a strict integrated approach using tandem mass spectra. This protein profiling method using gel electrophoresis coupled with nano-HPLC/ESI/MS/MS and manual evaluation is a sensitive and accurate method for protein identification as well as a powerful tool for monitoring various types of food products.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Dietary Proteins / analysis*
  • Electrophoresis, Polyacrylamide Gel
  • Food Analysis / methods
  • Infant Formula / chemistry*
  • Nanotechnology
  • Sensitivity and Specificity
  • Spectrometry, Mass, Electrospray Ionization / methods*
  • Tandem Mass Spectrometry / methods*

Substances

  • Dietary Proteins