A protocol for combined delipidation and subfractionation of membrane proteins using organic solvents

Anal Biochem. 2006 Oct 1;357(1):144-6. doi: 10.1016/j.ab.2006.05.035. Epub 2006 Jun 13.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chloroform / chemistry
  • Electrophoresis, Gel, Two-Dimensional
  • Humans
  • Lipids / chemistry*
  • Lipids / isolation & purification*
  • Lysosomes / chemistry
  • Membrane Proteins / chemistry
  • Membrane Proteins / isolation & purification*
  • Methanol / chemistry
  • Solvents / chemistry*
  • Trifluoroacetic Acid / chemistry

Substances

  • Lipids
  • Membrane Proteins
  • Solvents
  • Chloroform
  • Trifluoroacetic Acid
  • Methanol