Abstract
After growth of gonococci in the presence of cytidine monophospho-N-acetyl-neuraminic acid (CMP-NANA), their 4.5-kD lipooligosaccharide (LOS) component was increased by approximately 400 daltons, whereas the LOS of strains lacking the 4.5-kD component were unaffected. Expression of mAb-defined epitopes on the 4.5-kD component was decreased on LOS of strains grown in CMP-NANA, and treatment of the LOS with neuraminidase reversed this affect. Gonococci incubated with human PMNs also had decreased expression of the 4.5-kD+ epitopes. A detergent extract of gonococci incorporated radiolabeled NANA in the LOS, suggesting the presence of a sialyltransferase in gonococci. Exogenous sialyltransferases also could use LOS as an acceptor.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Monoclonal
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Antigens, Bacterial / immunology*
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Cytidine Monophosphate N-Acetylneuraminic Acid / metabolism*
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Electrophoresis, Polyacrylamide Gel
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Epitopes / immunology*
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Humans
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Immunoblotting
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Lipopolysaccharides / immunology*
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Lipopolysaccharides / isolation & purification
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Microscopy, Electron
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Neisseria gonorrhoeae / immunology*
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Neisseria gonorrhoeae / ultrastructure
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Neuraminidase
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Neutrophils / microbiology
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Radioimmunoassay
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Sialic Acids / metabolism*
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Submandibular Gland / enzymology
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Swine
Substances
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Antibodies, Monoclonal
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Antigens, Bacterial
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Epitopes
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Lipopolysaccharides
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Sialic Acids
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lipid-linked oligosaccharides
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Cytidine Monophosphate N-Acetylneuraminic Acid
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Neuraminidase