Autocrine effect of endothelin on DNA synthesis in human vascular endothelial cells

Biochem Biophys Res Commun. 1990 Apr 30;168(2):537-43. doi: 10.1016/0006-291x(90)92354-3.

Abstract

To elucidate the role of endogenous endothelin on DNA synthesis in endothelial cells, we have investigated the effects of rabbit anti-ET gamma globulin on DNA synthesis in cultured human vascular endothelial cells. DNA synthesis was markedly inhibited by anti-ET gamma globulin in a concentration dependent manner in the presence of fetal calf serum(FCS) (x5000;34%, x2500;54%, x1000;70%), but not in the absence of FCS. These data suggest that endogenous ET modulates FCS-stimulated DNA synthesis in an autocrine fashion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • DNA / biosynthesis*
  • Endothelins
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / metabolism*
  • Growth Substances*
  • Humans
  • Ionomycin / metabolism
  • Peptides / pharmacology*
  • Rabbits
  • Radioimmunoassay
  • Thrombin / metabolism
  • gamma-Globulins / genetics

Substances

  • Endothelins
  • Growth Substances
  • Peptides
  • gamma-Globulins
  • Ionomycin
  • DNA
  • Thrombin