Antibodies to the beta-amyloid peptide cross-react with conformational epitopes in human fibrinogen subunits from peripheral blood

FEBS Lett. 1990 May 7;264(1):43-7. doi: 10.1016/0014-5793(90)80760-g.

Abstract

Antibodies to the Alzheimer disease (AD) beta-amyloid peptide (beta AP) were used to identify beta AP precursor fragments in blood. The antibodies detected 3 major polypeptides with apparent molecular weights (MW) of 47-64,000 in Western blots of plasma derived clot proteins, but these proteins corresponded to human A-alpha, B-beta and gamma-fibrinogen since they reacted with 2 different anti-fibrinogen antisera, and the anti-beta AP and anti-fibrinogen antibodies recognized purified fibrinogen and fibrin. These data are significant for efforts to develop immunochemical assays to diagnose and monitor the progression of AD.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adrenal Gland Neoplasms / analysis
  • Alzheimer Disease / metabolism
  • Amyloid / analysis
  • Amyloid / immunology*
  • Amyloid beta-Peptides
  • Animals
  • Antibodies
  • Antibodies, Monoclonal
  • Blotting, Western
  • Brain Chemistry
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes / analysis*
  • Fibrinogen / immunology*
  • Humans
  • Nerve Tissue Proteins / immunology*
  • Pheochromocytoma / analysis
  • Protein Conformation
  • Rats

Substances

  • Amyloid
  • Amyloid beta-Peptides
  • Antibodies
  • Antibodies, Monoclonal
  • Epitopes
  • Nerve Tissue Proteins
  • Fibrinogen