A transgenic zebrafish model of neutrophilic inflammation

Blood. 2006 Dec 15;108(13):3976-8. doi: 10.1182/blood-2006-05-024075. Epub 2006 Aug 22.

Abstract

We have established an in vivo model for genetic analysis of the inflammatory response by generating a transgenic zebrafish line that expresses GFP under the neutrophil-specific myeloperoxidase promoter. We show that inflammation is induced after transection of the tail of zebrafish larvae and that this inflammation subsequently resolves over a similar time course to mammalian systems. Quantitative data can be generated from this model by counting of fluorescent cells or by digital image analysis. In addition, we show that the resolution of experimentally induced inflammation can be inhibited by the addition of a pancaspase inhibitor, zVD.fmk, demonstrating that experimental manipulation of the resolution of inflammation is possible in this model.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Cell Count / methods
  • Disease Models, Animal
  • Green Fluorescent Proteins
  • Image Processing, Computer-Assisted
  • Inflammation / genetics
  • Inflammation / immunology
  • Inflammation / pathology
  • Neutrophils / immunology*
  • Neutrophils / pathology
  • Peroxidase / genetics
  • Peroxidase / immunology
  • Promoter Regions, Genetic
  • Transgenes / immunology
  • Wounds and Injuries / genetics
  • Wounds and Injuries / immunology*
  • Wounds and Injuries / pathology
  • Zebrafish / genetics
  • Zebrafish / immunology*

Substances

  • Green Fluorescent Proteins
  • Peroxidase