Identification and molecular detection of a deletion mutation responsible for a truncated cadherin of Helicoverpa armigera

Yajun Yang; Haiyan Chen; Shuwen Wu; Yihua Yang; Xinjun Xu; Yidong Wu

doi:10.1016/j.ibmb.2006.06.003

Identification and molecular detection of a deletion mutation responsible for a truncated cadherin of Helicoverpa armigera

Insect Biochem Mol Biol. 2006 Sep;36(9):735-40. doi: 10.1016/j.ibmb.2006.06.003. Epub 2006 Jun 16.

Authors

Yajun Yang¹, Haiyan Chen, Shuwen Wu, Yihua Yang, Xinjun Xu, Yidong Wu

Affiliation

¹ Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, PR China.

PMID: 16935222
DOI: 10.1016/j.ibmb.2006.06.003

Abstract

Cadherins are a class of receptor proteins for Bacillus thuringiensis Cry1A toxins. Disruption of a cadherin gene (Ha_BtR) is associated with Cry1Ac resistance in the cotton bollworm Helicoverpa armigera [Xu, X., Yu, L., Wu, Y., 2005. Disruption of a cadherin gene associated with resistance to Cry1Ac delta-endotoxin of B. thuringiensis in H. armigera. Appl. Environ. Microbiol. 71, 948-954]. Determination of the genomic DNA sequences of Ha_BtR gene showed that the wild type Ha_BtR coding sequence is comprised of 34 exons. A deletion between Exon 8 and Exon 25 was found to be responsible for a truncated cadherin in the Cry1Ac-resistant GYBT strain of H. armigera. The mutant allele of Ha_BtR (r1) has two possible transcription variants, both of which produce the same truncated protein. A DNA-based detection method specific to the r1 allele was developed. This study will facilitate the monitoring of cadherin mutant frequency in field populations of H. armigera.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cadherins / genetics
Cadherins / isolation & purification*
Exons
Gene Deletion
Introns
Lepidoptera / genetics*
Mutation

Substances

Cadherins
H-cadherin