Abstract
A neutralization escape mutant (A/1 E) of equine infectious anemia virus was isolated after 13 passages in cell culture in the presence of serum containing antibodies to type- and group-specific determinants of EIAV envelope glycoproteins. Loss of neutralization by the selecting serum correlated with loss of two epitopes in the major envelope glycoprotein gp90 of A/1 E which were present in a parallel variant isolated from a persistently infected pony.
MeSH terms
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Animals
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Antibodies, Monoclonal
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Antibodies, Viral / immunology
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Antigens, Viral / genetics
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Antigens, Viral / immunology
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Cells, Cultured
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Electrophoresis, Polyacrylamide Gel
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Epitopes / immunology
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Glycoproteins / genetics
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Glycoproteins / immunology
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Immunoblotting
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Infectious Anemia Virus, Equine / genetics
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Infectious Anemia Virus, Equine / isolation & purification*
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Mutation*
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Neutralization Tests
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Serial Passage
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Viral Envelope Proteins / genetics
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Viral Envelope Proteins / immunology
Substances
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Antibodies, Monoclonal
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Antibodies, Viral
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Antigens, Viral
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Epitopes
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Glycoproteins
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Viral Envelope Proteins