In a 2-month survey of 186 children with gastro-enteritis attending an out-patient clinic in Malawi, 42% were infected with rotavirus (HRV), 9% Cryptosporidium, 4.2% adenovirus, 1.2% Astrovirus and 0.6% Norwalk agent and small round featureless viruses. We believe this to be the first report of HRV in Malawi and the first of Astrovirus in Africa. Almost all the HRV infections were in children under 12 months old, 40% were in children under 6 months and 64% of children were being breastfed at presentation. Signs of respiratory tract infection were not unique to HRV gastro-enteritis. Polyacrylamide gel electrophoresis of HRV dsRNA from 25 of the faecal samples revealed that each had the same long electropherotype.