The performance of a laboratory-scale upflow anaerobic sludge blanket (UASB) reactor treating distiller's grains wastewater was investigated for 420 days at 37 degrees C. After a successful start-up, 80-97.3% chemical oxygen demand (COD) removal efficiencies were achieved at hydraulic retention times (HRT) of 82-11h with organic loading rates (OLR) of 5-48.3kgCODm(-3)d(-1). The biogas mainly consisted of methane and carbon dioxide, and the methane and carbon dioxide content in the biogas was 57-60 and 38-41%, respectively. The yield coefficient of methane production was 0.3182lCH(4)g(-1)COD removed until OLR at 33.3kgCODm(-3)d(-1), but afterwards began to decrease. The volatile fatty acid (VFA) in the effluent mainly consisted of acetate and propionate, accounting for more than 95% of total VFA as COD, and other VFA was detected at insignificant concentrations. The mesophilic granules developed in this study showed an excellent specific methanogenic activity (SMA) at 0.91 and 1.12gmethaneCODg(-1)VSS(-1)d(-1) using sucrose and acetate as individual substrates on day 200, respectively.