Dietary flavonoids with a catechol structure increase alpha-tocopherol in rats and protect the vitamin from oxidation in vitro

J Lipid Res. 2006 Dec;47(12):2718-25. doi: 10.1194/jlr.M600291-JLR200. Epub 2006 Sep 1.

Abstract

To identify dietary phenolic compounds capable of improving vitamin E status, male Sprague-Dawley rats were fed for 4 weeks either a basal diet (control) with 2 g/kg cholesterol and an adequate content of vitamin E or the basal diet fortified with quercetin (Q), (-)-epicatechin (EC), or (+)-catechin (C) at concentrations of 2 g/kg. All three catechol derivatives substantially increased concentrations of alpha-tocopherol (alpha-T) in blood plasma and liver. To study potential mechanisms underlying the observed increase of alpha-T, the capacities of the flavonoids to i) protect alpha-T from oxidation in LDL exposed to peroxyl radicals, ii) reduce alpha-tocopheroxyl radicals (alpha-T (.) ) in SDS micelles, and iii) inhibit the metabolism of tocopherols in HepG2 cells were determined. All flavonoids protected alpha-T from oxidation in human LDL ex vivo and dose-dependently reduced the concentrations of alpha-T (.) . None of the test compounds affected vitamin E metabolism in the hepatocyte cultures. In conclusion, fortification of the diet of Sprague-Dawley rats with Q, EC, or C considerably improved their vitamin E status. The underlying mechanism does not appear to involve vitamin E metabolism but may involve direct quenching of free radicals or reduction of the alpha-T (.) by the flavonoids.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Catechols / chemistry
  • Diet
  • Flavonoids / chemistry*
  • Flavonoids / pharmacology*
  • Humans
  • In Vitro Techniques
  • Lipoproteins, LDL / chemistry
  • Lipoproteins, LDL / drug effects
  • Lipoproteins, LDL / metabolism
  • Liver / drug effects
  • Liver / metabolism
  • Male
  • Molecular Structure
  • Oxidation-Reduction
  • Rats
  • Rats, Sprague-Dawley
  • alpha-Tocopherol / blood
  • alpha-Tocopherol / chemistry
  • alpha-Tocopherol / metabolism*

Substances

  • Catechols
  • Flavonoids
  • Lipoproteins, LDL
  • alpha-Tocopherol
  • catechol