Isolation of respiratory syncytial virus from nasopharyngeal aspirates stored at 20 degrees C from one to fifteen months after collection

I F S Nunes; F E A Moura

doi:10.1590/s0074-02762006000400017

Isolation of respiratory syncytial virus from nasopharyngeal aspirates stored at 20 degrees C from one to fifteen months after collection

Mem Inst Oswaldo Cruz. 2006 Jun;101(4):451-3. doi: 10.1590/s0074-02762006000400017.

Authors

I F S Nunes¹, F E A Moura

Affiliation

¹ Laboratório de Virologia, Departamento de Patologia e Medicina Legal, Universidade Federal do Ceará, Fortaleza, CE, 60441-750, Brasil.

PMID: 16951818
DOI: 10.1590/s0074-02762006000400017

Abstract

Cell culture isolation is used for recovering respiratory syncytial virus (RSV) from respiratory specimens. As RSV is a thermolabile virus, specimens destined for inoculation into cell culture require special transport, handling, and storage. The isolation rate of RSV from nasopharyngeal aspirates (NPA) stored at 20 20 degrees C for one to 15 months after collection was investigated. A total of 126 samples considered positive for RSV by indirect fluorescence-antibody were tested by virus isolation in HEp-2 cell culture. RSV was isolated from 47/126 specimens (37.3%). These results show that RSV may be recovered from NPA stored at 20 20 degrees C by cell culture.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Cryopreservation / methods
Fluorescent Antibody Technique
Humans
Nasopharynx / virology*
Respiratory Syncytial Virus Infections / virology*
Respiratory Syncytial Viruses / isolation & purification*
Time Factors