Diagnosis of gastric syphilis by direct immunofluorescence staining and real-time PCR testing

J Clin Microbiol. 2006 Sep;44(9):3452-6. doi: 10.1128/JCM.00721-06.

Abstract

We report on a case of gastric syphilis in a patient with chronic dyspepsia. The diagnosis was established by serology and the demonstration of spirochetes in diffusely inflammed gastric mucosa by staining with a fluorescent monoclonal antibody specific for pathogenic treponemes and by the detection of specific treponemal DNA sequences by a real-time PCR.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • DNA, Bacterial / analysis
  • Fluorescent Antibody Technique, Direct / methods*
  • Gastric Mucosa / microbiology
  • Humans
  • Male
  • Polymerase Chain Reaction / methods*
  • Staining and Labeling / methods
  • Stomach Diseases / diagnosis*
  • Stomach Diseases / microbiology*
  • Syphilis / diagnosis*
  • Syphilis / microbiology
  • Treponema pallidum / genetics
  • Treponema pallidum / isolation & purification

Substances

  • DNA, Bacterial