High-performance liquid chromatography analysis of rubber allergens in protective gloves used in health care

Contact Dermatitis. 2006 Oct;55(4):210-5. doi: 10.1111/j.1600-0536.2006.00912.x.

Abstract

A high-performance liquid chromatography (HPLC) method developed for analysis of zinc dithiocarbamates was validated and used to perform a survey of disposable medical gloves used in southern Sweden. The gloves were extracted with acetone at room temperature for 10 min by shaking. The extracts were injected into a polyether ether ketone lined HPLC column, and peaks were analysed by a diode-array detector. The survey shows that of 19 gloves analysed, 10 contained zinc diethyldithiocarbamate (0.070-3.5 mg/g), 3 contained zinc pentamethylenedithiocarbamate (1.0-4.3 mg/g), 4 contained zinc dibutyldithiocarbamate (0.9-1.1 mg/g), and 2 contained 2-mercaptobenzothiazole (0.005-0.008 mg/g). None of them contained thiurams.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Allergens / chemistry*
  • Chromatography, High Pressure Liquid / methods*
  • Dermatitis, Occupational / prevention & control*
  • Gloves, Protective*
  • Hand Dermatoses / prevention & control*
  • Health Personnel
  • Humans
  • Latex Hypersensitivity / prevention & control*
  • Predictive Value of Tests
  • Rubber / chemistry*
  • Thiocarbamates / chemistry

Substances

  • Allergens
  • Thiocarbamates
  • Rubber
  • zinc dibutyldithiocarbamate