Hodgkin lymphoma cells express TACI and BCMA receptors and generate survival and proliferation signals in response to BAFF and APRIL

Blood. 2007 Jan 15;109(2):729-39. doi: 10.1182/blood-2006-04-015958. Epub 2006 Sep 7.

Abstract

Hodgkin lymphoma (HL) originates from the clonal expansion of malignant Hodgkin and Reed-Sternberg (HRS) cells. These B-cell-derived elements constitute less than 10% of the tumoral mass. The remaining tissue is comprised of an inflammatory infiltrate that includes myeloid cells. Myeloid cells activate B cells by producing BAFF and APRIL, which engage TACI, BCMA, and BAFF-R receptors on the B cells. Here, we studied the role of BAFF and APRIL in HL. Inflammatory and HRS cells from HL tumors expressed BAFF and APRIL. Unlike their putative germinal center B-cell precursors, HRS cells lacked BAFF-R, but expressed TACI and BCMA, a phenotype similar to that of plasmacytoid B cells. BAFF and APRIL enhanced HRS cell survival and proliferation by delivering nonredundant signals via TACI and BCMA receptors through both autocrine and paracrine pathways. These signals caused NF-kappaB activation; Bcl-2, Bcl-xL, and c-Myc up-regulation; and Bax down-regulation, and were amplified by APRIL-binding proteoglycans on HRS cells. Interruption of BAFF and APRIL signaling by TACI-Ig decoy receptor, which binds to and neutralizes BAFF and APRIL, or by small-interfering RNAs targeting BAFF, APRIL, TACI, and BCMA inhibited HRS cell accumulation in vitro and might attenuate HL expansion in vivo.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • B-Cell Activating Factor / metabolism*
  • B-Cell Activating Factor / pharmacology
  • B-Cell Maturation Antigen / biosynthesis*
  • B-Cell Maturation Antigen / genetics
  • B-Lymphocytes / immunology*
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Gene Expression Profiling
  • Hodgkin Disease / diagnosis
  • Hodgkin Disease / metabolism*
  • Hodgkin Disease / pathology
  • Humans
  • Immunophenotyping
  • RNA Interference
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Signal Transduction
  • Transmembrane Activator and CAML Interactor Protein / biosynthesis*
  • Transmembrane Activator and CAML Interactor Protein / genetics
  • Tumor Necrosis Factor Ligand Superfamily Member 13 / metabolism*
  • Tumor Necrosis Factor Ligand Superfamily Member 13 / pharmacology

Substances

  • B-Cell Activating Factor
  • B-Cell Maturation Antigen
  • TNFSF13 protein, human
  • TNFSF13B protein, human
  • Transmembrane Activator and CAML Interactor Protein
  • Tumor Necrosis Factor Ligand Superfamily Member 13