Abstract
Peptides in bee venom (PBV) have attracted considerable attention for anti-cancer therapy. In this study, sterically stabilized liposomal PBV (PBV-SL) was prepared using Soybean phosphatidylcholine, cholesterol, and the cholesterol derivatives of PEG with terminal COOH groups. The humanized anti-hepatoma disulfide-stabilized Fv (hdsFv25) was coupled to sterically stabilized liposomes. The hdsFv25-immunoliposome has strong affinity and specificity to SMMC-7721 cells in vitro. PBV-loaded sterically stabilized liposomes modified with the hdsFv25 can kill SMMC-7721 cells in vitro with higher efficiency than non-targeted liposomes. These results demonstrate that this strategy should also be applicable to immunotherapy for other cancers.
MeSH terms
-
Antineoplastic Agents / chemistry*
-
Antineoplastic Agents / pharmacology
-
Bee Venoms / chemistry*
-
Bee Venoms / pharmacology
-
Carcinoma, Hepatocellular / drug therapy
-
Carcinoma, Hepatocellular / pathology
-
Cell Line, Tumor
-
Cell Survival
-
Cholesterol / chemistry
-
Drug Delivery Systems*
-
Drug Screening Assays, Antitumor
-
HeLa Cells
-
Humans
-
Immunoconjugates / chemistry*
-
Immunoconjugates / pharmacology
-
Liposomes*
-
Microscopy, Fluorescence
-
Particle Size
-
Polyethylene Glycols / chemistry*
-
Recombinant Proteins / administration & dosage
-
Recombinant Proteins / chemistry
-
Recombinant Proteins / pharmacology
-
Tetrazolium Salts
-
Thiazoles
Substances
-
Antineoplastic Agents
-
Bee Venoms
-
Immunoconjugates
-
Liposomes
-
Recombinant Proteins
-
Tetrazolium Salts
-
Thiazoles
-
hdsFv25, human
-
Polyethylene Glycols
-
Cholesterol
-
thiazolyl blue