Abstract
We have previously reported artificial gene-regulation systems responding to cyclic AMP-dependent protein kinase (PKA) using a cationic polymer. However, this polymer alone cannot deliver any gene into living cells. In the present work, we modified the signal-responsive polymer to the RGD peptide for the introduction of a polymer/DNA complex into living cells and succeeded in regulating the gene expression responding to intracellular PKA activation.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Cell Line, Tumor
-
DNA / genetics
-
DNA / metabolism
-
Enzyme Activation
-
Gene Expression Regulation / drug effects*
-
Genetic Therapy / methods*
-
HeLa Cells
-
Humans
-
Integrins / drug effects*
-
Integrins / genetics
-
Integrins / metabolism
-
Luciferases / genetics
-
Luciferases / metabolism
-
Oligopeptides / chemical synthesis
-
Oligopeptides / pharmacology*
-
Protein Kinases / genetics*
-
Protein Kinases / metabolism
-
Signal Transduction / drug effects*
Substances
-
Integrins
-
Oligopeptides
-
arginyl-glycyl-aspartic acid
-
DNA
-
Luciferases
-
Protein Kinases