Abstract
We developed a rapid genetic approach for screening bfpA variants of enteropathogenic E. coli(EPEC) using a heteroduplex mobility assay (HMA). A total of 204 human EPEC strains were isolated in Thailand and Japan. Of 34 bfpA-positive EPEC strains, bfpA variants were classified into 5 HMA-types. Different HMA-types were found in EPEC of the same serotypes. The results suggest that HMA is a simple and easy method to analyze polymorphism of bfpA gene, and can be used in laboratories without large apparatus such as sequencers.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Base Sequence
-
Escherichia coli / classification*
-
Escherichia coli / genetics*
-
Escherichia coli Infections / microbiology*
-
Escherichia coli Proteins / genetics*
-
Fimbriae Proteins / genetics*
-
Heteroduplex Analysis / methods*
-
Humans
-
Japan
-
Molecular Sequence Data
-
Phylogeny
-
Sequence Alignment
-
Thailand
Substances
-
BfpA protein, E coli
-
Escherichia coli Proteins
-
Fimbriae Proteins
Associated data
-
GENBANK/AB247922
-
GENBANK/AB247923
-
GENBANK/AB247924
-
GENBANK/AB247925
-
GENBANK/AB247926
-
GENBANK/AB247927
-
GENBANK/AB247928
-
GENBANK/AB247929
-
GENBANK/AB247930
-
GENBANK/AB247931
-
GENBANK/AB247932
-
GENBANK/AB247933
-
GENBANK/AB247934
-
GENBANK/AB247935