Abstract
A nuclear factor I (NFI) binding sequence in the myelin basic protein (MBP) promoter is required for brain-specific transcription. In the present study, we isolated multiple cDNAs encoding a family of NFI, NFI-B, from mouse brain cDNA libraries. These clones contain unique nucleotide sequences at their 5', 3', or middle portions, presumably as a result of alternative splicing. The entire sequence of NFI-B suggests that NFI-B is a mouse counterpart of NFI-L, which was previously isolated from rat liver. NFI-B is highly enriched in the cerebellum, in contrast with a ubiquitous NFI, CTF/NF-I. NFI-B proteins translated in vitro could bind to the MBP NFI site. Furthermore, we demonstrated by transfection experiments that NFI-B activated transcription from transfected MBP promoter in neuronal/glial NG108-15 cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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CCAAT-Enhancer-Binding Proteins*
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Cell Line
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Cerebellum / metabolism*
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Cloning, Molecular
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DNA / genetics*
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DNA / isolation & purification
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Gene Expression Regulation*
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Gene Library
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Mice
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Mice, Inbred ICR
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Molecular Sequence Data
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Myelin Basic Protein / genetics*
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NFI Transcription Factors
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Nuclear Proteins
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Promoter Regions, Genetic*
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Protein Binding
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Transcription Factors*
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Transcription, Genetic*
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Transfection
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Y-Box-Binding Protein 1
Substances
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CCAAT-Enhancer-Binding Proteins
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DNA-Binding Proteins
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Myelin Basic Protein
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NFI Transcription Factors
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Nfic protein, mouse
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Nuclear Proteins
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Transcription Factors
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Y-Box-Binding Protein 1
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YBX1 protein, human
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DNA
Associated data
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GENBANK/D90172
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GENBANK/D90173
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GENBANK/D90174
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GENBANK/D90175
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GENBANK/D90176