Abstract
Construction and integration of recombinant mini-Tn7 expression vectors into the chromosome of a surrogate, efflux-sensitized, and biosafe Pseudomonas aeruginosa host was validated as a generally applicable method for studies of uncharacterized bacterial efflux pumps. Using this method, the Burkholderia pseudomallei bpeEF-oprC operon was shown to encode a chloramphenicol and trimethoprim efflux pump.
Publication types
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Evaluation Study
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Research Support, N.I.H., Extramural
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Burkholderia pseudomallei / drug effects*
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Burkholderia pseudomallei / genetics
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Carrier Proteins / genetics
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Carrier Proteins / metabolism
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Chloramphenicol / pharmacology
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DNA Transposable Elements
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Drug Resistance, Multiple, Bacterial*
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Gene Expression Regulation, Bacterial*
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Genetic Techniques*
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Genetic Vectors*
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Genome, Bacterial
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Microbial Sensitivity Tests
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Pseudomonas aeruginosa / genetics
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Pseudomonas aeruginosa / metabolism*
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Recombination, Genetic
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Trimethoprim / pharmacology
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Carrier Proteins
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DNA Transposable Elements
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Chloramphenicol
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Trimethoprim