Gene-gene interaction between IL-13 and IL-13Ralpha1 is associated with total IgE in Korean children with atopic asthma

J Hum Genet. 2006;51(12):1055-1062. doi: 10.1007/s10038-006-0061-x. Epub 2006 Sep 28.

Abstract

Interleukin (IL)-13, which is essential for IgE synthesis, mediates its effects by binding with a receptor composed of IL-4Ralpha and IL-13Ralpha1. We investigated the effects of IL-13 and IL-13Ralpha1 polymorphisms in Korean children with asthma, and whether these have been associated with IgE production. We enrolled 358 atopic asthmatic, 111 non-atopic asthmatic, and 146 non-atopic healthy children. IL-13 and IL-13Ralpha1 genotypes were identified using the PCR-RFLP method. There was an association between the asthma susceptibility and homozygosity for risk allele of IL-13 G+2044A. In children with atopic asthma, risk alleles in IL-13 (A-1512C and C-1112T) and IL-13Ralpha1 (A+1398G) showed increased total IgE (P=0.012, 0.015 and 0.017, respectively). Three-loci haplotype analysis for IL-13 showed that the haplotype composed of -1512C, -1112T and +2044A was associated with higher total IgE than other tested haplotypes in children with atopic asthma (P=0.003). The gene-gene interaction between risk alleles of each IL-13 promoter polymorphism and IL-13Ralpha1 polymorphism was associated with higher total IgE in children with atopic asthma (P=0.002, 0.010). These findings indicate that the IL-13 G+2044A is associated with asthma development and the IL-13 and IL-13Ralpha1 polymorphisms may interact to enhance IgE production.

MeSH terms

  • Asian People
  • Asthma / genetics*
  • Asthma / immunology
  • Child
  • Female
  • Gene Expression Regulation
  • Genetic Predisposition to Disease
  • Genotype
  • Haplotypes
  • Humans
  • Immunoglobulin E / blood*
  • Interleukin-13 / genetics*
  • Interleukin-13 / metabolism
  • Interleukin-13 Receptor alpha1 Subunit / genetics*
  • Interleukin-13 Receptor alpha1 Subunit / metabolism
  • Male
  • Polymorphism, Genetic*
  • Promoter Regions, Genetic
  • Respiratory Hypersensitivity / genetics
  • Respiratory Hypersensitivity / immunology

Substances

  • Interleukin-13
  • Interleukin-13 Receptor alpha1 Subunit
  • Immunoglobulin E