Abstract
Activity of matrix metalloprotease-2 (MMP-2) and the expression of its related molecules were examined in spontaneous canine oronasal tumors. Tissue samples from melanoma and squamous cell carcinoma possessed higher MMP-2 activity, as shown in gelatin zymography, in comparison with acanthomatous epulis and nasal adenocarcinoma. Regional lymph node invasion and distant metastases were more frequently observed in the MMP-2 positive cases. There were no significant differences by RT-PCR examination in the expression of the genes encoding MMP-2, MT1-MMP and TIMP-2 among the tumor histological types. However, the MMP-2/TIMP-2 ratio showed a significantly higher level of the genes in the malignant oral melanoma and squamous cell carcinoma. The MMP-2/TIMP-2 ratio was also positively correlated with MMP-2 activity in gelatin zymography. These results indicate that the MMP-2/TIMP-2 ratio may be of value in evaluating the prognosis in canine oronasal cavity tumors.
MeSH terms
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Animals
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Carcinoma, Squamous Cell / enzymology
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Carcinoma, Squamous Cell / pathology
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Carcinoma, Squamous Cell / veterinary*
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Dog Diseases / enzymology*
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Dog Diseases / pathology
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Dogs
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Electrophoresis, Polyacrylamide Gel / veterinary
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Female
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Lymphatic Metastasis
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Male
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Matrix Metalloproteinase 14 / biosynthesis
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Matrix Metalloproteinase 14 / genetics
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Matrix Metalloproteinase 14 / metabolism
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Matrix Metalloproteinase 2 / biosynthesis
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Matrix Metalloproteinase 2 / genetics
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Matrix Metalloproteinase 2 / metabolism*
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Melanoma / enzymology
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Melanoma / pathology
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Melanoma / veterinary*
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Mouth Neoplasms / enzymology
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Mouth Neoplasms / pathology
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Mouth Neoplasms / veterinary*
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Nose Neoplasms / enzymology
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Nose Neoplasms / pathology
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Nose Neoplasms / veterinary*
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Reverse Transcriptase Polymerase Chain Reaction / veterinary
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Statistics, Nonparametric
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Tissue Inhibitor of Metalloproteinase-2 / biosynthesis
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Tissue Inhibitor of Metalloproteinase-2 / genetics
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Tissue Inhibitor of Metalloproteinase-2 / metabolism
Substances
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RNA, Messenger
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Tissue Inhibitor of Metalloproteinase-2
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Matrix Metalloproteinase 2
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Matrix Metalloproteinase 14