Abstract
C-phycocyanin (C-PC) was extracted from fresh Spirulina platensis by deploying a species of non-pathogenic nitrogen-fixing bacteria, namely, Klebsiella pneumoniae. The algal slurry was neither washed nor centrifuged; the bacterial culture was poured into the slurry, the vessel sealed, and crude C-PC extracted after about 24 h. The extraction was clean and efficient, and the purity and concentration of C-PC proved to be of adequate quality.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / chemistry*
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Bacteriolysis*
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Culture Media
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Freezing
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Glass
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Industrial Microbiology / methods
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Klebsiella pneumoniae / growth & development*
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Klebsiella pneumoniae / physiology
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Microspheres
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Muramidase / metabolism
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Nitrogen Fixation
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Phycocyanin / isolation & purification*
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Sonication
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Spirulina / chemistry*
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Spirulina / growth & development
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Spirulina / physiology*
Substances
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Bacterial Proteins
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Culture Media
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Phycocyanin
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Muramidase