Abstract
We investigated hepatoprotective activity and antioxidant effect of the 2,5-dihydroxy-4,3'-di(beta-D-glucopyranosyloxy)-trans-stilbene that purified from Morus bombycis Koidzumi roots against CCl4-induced liver damage in rats. The 2,5-dihydroxy-4,3'-di(beta-D-glucopyranosyloxy)-trans-stilbene displayed dose-dependent superoxide radical scavenging activity (IC50 = 430.2 microg/ml), as assayed by the electron spin resonance (ESR) spin-trapping technique. The increase in aspartate aminotransferase (AST) activities in serum associated with carbon tetrachloride (CCl4)-induced liver injury was inhibited by 2,5-dihydroxy-4,3'-di(beta-D-glucopyranosyloxy)-trans-stilbene and at a dose of 400 - 600 mg/kg samples had hepatoprotective activity comparable to the standard agent, silymarin. The biochemical assays were confirmed by histological observations showing that the 2,5-dihydroxy-4,3'-di(beta-d-glucopyranosyloxy)-trans-stilbene decreased cell ballooning in response to CCl4 treatment. These results demonstrate that the 2,5-dihydroxy-4,3'-di(beta-D-glucopyranosyloxy)-trans-stilbene is a potent antioxidant with a liver protective action against CCl4-induced hepatotoxicity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antioxidants / chemistry
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Antioxidants / isolation & purification
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Antioxidants / pharmacology*
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Carbon Tetrachloride / toxicity
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Chemical and Drug Induced Liver Injury / pathology
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Chemical and Drug Induced Liver Injury / prevention & control*
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Cytochrome P-450 CYP2E1 / biosynthesis
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Cytochrome P-450 CYP2E1 / drug effects
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Dose-Response Relationship, Drug
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Free Radical Scavengers / chemistry
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Free Radical Scavengers / isolation & purification
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Free Radical Scavengers / pharmacology*
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Liver / drug effects*
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Liver / metabolism
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Liver / pathology
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Male
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Morus / chemistry*
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology
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Plant Roots / chemistry
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Plants, Medicinal / chemistry*
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Rats
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Rats, Wistar
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Silymarin / pharmacology
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Stilbenes / chemistry
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Stilbenes / pharmacology*
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Structure-Activity Relationship
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Superoxides / antagonists & inhibitors
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Tumor Necrosis Factor-alpha / biosynthesis
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Tumor Necrosis Factor-alpha / drug effects
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Wound Healing
Substances
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2,5-dihydroxy-4,3'-di(beta-d-glucopyranosyloxy)-trans-stilbene
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Antioxidants
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Free Radical Scavengers
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Plant Extracts
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Silymarin
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Stilbenes
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Tumor Necrosis Factor-alpha
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Superoxides
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Carbon Tetrachloride
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Cytochrome P-450 CYP2E1