Objective: To study the expression of Neuropilin-1 in human carcinoma and papilloma of larynx specimens and carcinoma cell lines Hep-2.
Method: The expression of Neuropilin-1 in human laryngeal carcinoma, papilloma specimens were investigated by immunohistochemical staining; the expression of Neuropilin-1 mRNA in human laryngeal squamous cell carcinoma, papilloma specimens and cell lines were examined by using RT-PCR.
Result: Immunohistochemical analysis revealed that NRP-1 was expressed in all human laryngeal squamous cell carcinoma specimens but was absent in nonmalignant laryngeal tissue. The mRNA of NRP land Hep-2 cell lines are positive in 24 of 28 human laryngeal carcinoma specimens but not negative in nonmalignant laryngeal specimens.
Conclusion: Neuropilin-1 was expressed in most of human laryngeal carcinoma specimens and cell lines but not expressed in nonmalignant tissue. It suggests that the expression of Neuropilin-1 may be related to the risk and development of laryngeal carcinoma and has high distinguishability.