Objective: To determine the content of notoginsenoside R1 and ginsenoside Rg1 in Rupixiao tablet by reverse-phase high performance liquid chromatography.
Method: A Kromacil C18 column was used with a mixture of acetonitrile and 0.05% phosphoric acid solution (20:80) as the mobile phase at a flow rate of 1.2 mL x min(-1) with the detection wavelength at 203 nm.
Result: The measurement proved to be linear over the range of 0.941-9.41 g for notoginsenoside R1 and 1.04-10.4 g for ginsenoside Rg1. The average recovery of this method was 97.3% and 97.9% respectively.
Conclusion: The method was simple, reliable, and accurate and can be used for the quality control of this preparation.