Abstract
DC-LAMP is a molecule expressed in mature dendritic cells, but its mRNA is also found in the lung. This study compares the immunostaining spectrum of PE-10, an antisurfactant protein monoclonal antibody; thyroid transcription factor-1 (TTF-1); and DC-LAMP in normal and neoplastic lung in an attempt to characterize the cell type(s) that express DC-LAMP. Electron microscopy was used to define cell types. DC-LAMP marks pulmonary adenocarcinomas that show Clara cell characteristics by electron microscopy. In contrast, PE-10 labels tumors that have Clara cell and type II pneumocyte differentiation. DC-LAMP staining was lost in solid type adenocarcinomas but persisted in well-differentiated areas. CC-10, an antibody that marks Clara cells, was also positive in tumors that labeled for DC-LAMP. There was no prognostic difference in tumors that reacted with DC-LAMP. DC-LAMP and CC-10 reactivity was also observed in endometrial adenocarcinomas but not in other tumor types.
MeSH terms
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Adenocarcinoma / metabolism
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Adenocarcinoma / mortality
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Adenocarcinoma / pathology*
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Aged
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Aged, 80 and over
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Antibodies, Monoclonal / immunology
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Bronchi / chemistry
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Bronchi / pathology*
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Bronchi / ultrastructure
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Carcinoma, Small Cell / metabolism
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Carcinoma, Small Cell / pathology
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Carcinoma, Squamous Cell / metabolism
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Carcinoma, Squamous Cell / pathology
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Cell Differentiation
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Endometrial Neoplasms / metabolism
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Endometrial Neoplasms / pathology
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Female
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Humans
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Immunohistochemistry
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Lung / chemistry
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Lung / pathology
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Lung / ultrastructure
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Lung Neoplasms / metabolism
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Lung Neoplasms / mortality
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Lung Neoplasms / pathology*
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Lysosomal Membrane Proteins / analysis*
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Lysosomal Membrane Proteins / immunology
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Male
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Microscopy, Electron
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Middle Aged
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Nuclear Proteins / analysis
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Prognosis
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Pulmonary Surfactants / analysis
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Survival Rate
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Thyroid Nuclear Factor 1
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Transcription Factors / analysis
Substances
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Antibodies, Monoclonal
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Lysosomal Membrane Proteins
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NKX2-1 protein, human
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Nuclear Proteins
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Pulmonary Surfactants
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Thyroid Nuclear Factor 1
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Transcription Factors