Abstract
We generated four individual glutamine synthetase (GS) mutants (DeltaglnA1, DeltaglnA2, DeltaglnA3, and DeltaglnA4) and one triple mutant (DeltaglnA1EA2) of Mycobacterium tuberculosis to investigate the roles of GS enzymes. Subcutaneous immunization with the DeltaglnA1EA2 and DeltaglnA1 glutamine auxotrophic mutants conferred protection on C57BL/6 mice against an aerosol challenge with virulent M. tuberculosis, which was comparable to that provided by Mycobacterium bovis BCG vaccination.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Disease Models, Animal
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Glutamate-Ammonia Ligase / genetics*
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Glutamate-Ammonia Ligase / physiology
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Glutamine / metabolism*
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Mice, SCID
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Mutation
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Mycobacterium tuberculosis / enzymology
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Mycobacterium tuberculosis / genetics*
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Mycobacterium tuberculosis / growth & development*
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Phenotype
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Tuberculosis, Pulmonary / enzymology
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Tuberculosis, Pulmonary / microbiology*
Substances
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Glutamine
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Glutamate-Ammonia Ligase