Abstract
MyoD mRNA is expressed in a subpopulation of cells within the embryonic epiblast. Most of these cells are incorporated into somites and synthesize Noggin. Ablation of MyoD-positive cells in the epiblast subsequently results in the herniation of organs through the ventral body wall, a decrease in the expression of Noggin, MyoD, Myf5, and myosin in the somites and limbs, and an increase in Pax-3-positive myogenic precursors. The addition of Noggin lateral to the somites compensates for the loss of MyoD-positive epiblast cells. Skeletal muscle stem cells that arise in the epiblast are utilized in the somites to promote muscle differentiation by serving as a source of Noggin.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Carrier Proteins / metabolism
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Cell Differentiation / physiology*
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Chick Embryo
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Embryo, Mammalian / cytology*
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Embryo, Mammalian / physiology
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Embryo, Nonmammalian*
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Epithelium / anatomy & histology
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Epithelium / physiology*
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Extremities
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Fluorescent Antibody Technique
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Gene Expression Regulation, Developmental
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In Situ Hybridization
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Morphogenesis
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Muscle, Skeletal / cytology*
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Muscle, Skeletal / embryology
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Muscle, Skeletal / metabolism
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MyoD Protein / genetics
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MyoD Protein / physiology*
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Myogenic Regulatory Factor 5 / metabolism
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Myosins / metabolism
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Paired Box Transcription Factors / metabolism
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Somites / metabolism
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Stem Cells / chemistry
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Stem Cells / cytology
Substances
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Carrier Proteins
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MyoD Protein
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Myogenic Regulatory Factor 5
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Paired Box Transcription Factors
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noggin protein
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Myosins