Abstract
Filoviruses have the capacity to cause lethal outbreaks of hemorrhagic fever in primates. Here we present a simple consensus reverse transcription-PCR method for filovirus recognition and characterization and demonstrate its utility with all known filovirus strains. Phylogenetic assignment is achieved by automated web-based sequence analysis of amplification products.
Publication types
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Evaluation Study
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Ebolavirus / genetics
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Ebolavirus / isolation & purification
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Filoviridae / classification*
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Filoviridae / genetics
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Filoviridae / isolation & purification*
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Filoviridae Infections / diagnosis*
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Filoviridae Infections / virology*
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Hemorrhagic Fever, Ebola
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Humans
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Marburg Virus Disease
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Marburgvirus / genetics
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Marburgvirus / isolation & purification
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Phylogeny
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Reverse Transcriptase Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
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Time Factors