Analysis of the Saccharomyces cerevisiae proteome with PeptideAtlas

Genome Biol. 2006;7(11):R106. doi: 10.1186/gb-2006-7-11-r106.

Abstract

We present the Saccharomyces cerevisiae PeptideAtlas composed from 47 diverse experiments and 4.9 million tandem mass spectra. The observed peptides align to 61% of Saccharomyces Genome Database (SGD) open reading frames (ORFs), 49% of the uncharacterized SGD ORFs, 54% of S. cerevisiae ORFs with a Gene Ontology annotation of 'molecular function unknown', and 76% of ORFs with Gene names. We highlight the use of this resource for data mining, construction of high quality lists for targeted proteomics, validation of proteins, and software development.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Codon
  • Databases, Protein*
  • Genes, Fungal
  • Hydrophobic and Hydrophilic Interactions
  • Mass Spectrometry
  • Molecular Weight
  • Open Reading Frames / genetics
  • Peptides / chemistry
  • Peptides / metabolism*
  • Proteome / chemistry
  • Proteome / genetics
  • Proteome / metabolism*
  • Saccharomyces cerevisiae / chemistry
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / chemistry
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism*
  • User-Computer Interface

Substances

  • Codon
  • Peptides
  • Proteome
  • Saccharomyces cerevisiae Proteins