Validation of a Rickettsia prowazekii-specific quantitative real-time PCR cassette and DNA extraction protocols using experimentally infected lice

Patrick J Rozmajzl; Linda Houhamdi; Ju Jiang; Didier Raoult; Allen L Richards

doi:10.1196/annals.1374.036

Validation of a Rickettsia prowazekii-specific quantitative real-time PCR cassette and DNA extraction protocols using experimentally infected lice

Ann N Y Acad Sci. 2006 Oct:1078:617-9. doi: 10.1196/annals.1374.036.

Authors

Patrick J Rozmajzl¹, Linda Houhamdi, Ju Jiang, Didier Raoult, Allen L Richards

Affiliation

¹ NMRC-IDD/RDD, 503 Robert Grant Ave., Silver Spring, MD 20910-7500, USA. [email protected]

PMID: 17114790
DOI: 10.1196/annals.1374.036

Abstract

The presence of R. prowazekii in lice using R. prowazekii-specific qPCR cassettes showed the changes of the rickettsial burden in the lice post-infection.

MeSH terms

Animals
DNA, Bacterial / genetics
DNA, Bacterial / isolation & purification
Disease Models, Animal
Phthiraptera / microbiology*
Polymerase Chain Reaction / methods*
Rickettsia prowazekii / genetics*
Rickettsia prowazekii / isolation & purification
Typhus, Epidemic Louse-Borne / diagnosis*

Substances

DNA, Bacterial