Estimation of the chloramphenicol and cycloheximide inhibition of protein synthesis in brain cholinergic synaptosomes

V Corbaton; M T Muiño; P Fernández-Silva; M J López-Pérez; J Montoya

doi:10.1016/0006-8993(91)90049-2

Estimation of the chloramphenicol and cycloheximide inhibition of protein synthesis in brain cholinergic synaptosomes

Brain Res. 1991 Mar 15;543(2):351-3. doi: 10.1016/0006-8993(91)90049-2.

Authors

V Corbaton¹, M T Muiño, P Fernández-Silva, M J López-Pérez, J Montoya

Affiliation

¹ Departamento de Bioqumica, Biología Molecular y Celular, Facultad de Veterinaria, Zaragoza, Spain.

PMID: 1711913
DOI: 10.1016/0006-8993(91)90049-2

Abstract

Cholinergic synaptosomes have been prepared from sheep brain cortex by means of an immunoaffinity method using a specific anti-(Chol I) antiserum. The [14C]leucine incorporation into proteins of this preparation shows a low cycloheximide and a high chloramphenicol sensitivity. This fact suggests that the mitochondrial protein synthesis system is the only one present in this fraction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain Chemistry / drug effects
Chloramphenicol / pharmacology*
Cycloheximide / pharmacology*
In Vitro Techniques
Leucine / metabolism
Mitochondria / drug effects
Mitochondria / metabolism
Nerve Tissue Proteins / biosynthesis*
Parasympathetic Nervous System / drug effects
Parasympathetic Nervous System / metabolism*
RNA / metabolism
Sheep
Synaptosomes / drug effects
Synaptosomes / metabolism*

Substances

Nerve Tissue Proteins
RNA
Chloramphenicol
Cycloheximide
Leucine