[Assessment of two methods of antigenic detection by ELISA for the diagnosis of norovirus outbreaks]

Enferm Infecc Microbiol Clin. 2006 Nov;24(9):564-7. doi: 10.1157/13093877.
[Article in Spanish]

Abstract

Introduction: The aim of this study was to assess two ELISA techniques for the detection of outbreaks of norovirus.

Method: One-hundred and sixty-five fecal samples from patients affected in 30 outbreaks were studied.

Results: On the basis of a specific consensus criterion between techniques for confirming outbreaks, the sensitivity and specificity was respectively 80% and 90% for RIDASCREEN, and 80% and 100% for IDEIA.

Conclusion: These techniques could be useful for rapid detection of norovirus outbreaks in laboratories that lack molecular confirmation techniques.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Caliciviridae Infections / diagnosis
  • Caliciviridae Infections / epidemiology
  • Caliciviridae Infections / virology*
  • Child
  • Child Day Care Centers
  • Child, Preschool
  • Disease Outbreaks
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Feces / virology*
  • Gastroenteritis / diagnosis
  • Gastroenteritis / epidemiology
  • Gastroenteritis / virology*
  • Hospitalization
  • Humans
  • Norovirus
  • Nursing Homes
  • Reproducibility of Results
  • Restaurants
  • Schools
  • Sensitivity and Specificity
  • Spain / epidemiology