The human CMV-UL86 peptide 981-1003 shares a crossreactive T-cell epitope with the encephalitogenic MOG peptide 34-56, but lacks the capacity to induce EAE in rhesus monkeys

Herbert P M Brok; Leonie Boven; Marjan van Meurs; Nicole Kerlero de Rosbo; Liesbeth Celebi-Paul; Yolanda S Kap; Anwar Jagessar; Rogier Q Hintzen; Geoff Keir; Jeffrey Bajramovic; Avraham Ben-Nun; Jan Bauer; Jon D Laman; Sandra Amor; Bert A 't Hart

doi:10.1016/j.jneuroim.2006.10.010

The human CMV-UL86 peptide 981-1003 shares a crossreactive T-cell epitope with the encephalitogenic MOG peptide 34-56, but lacks the capacity to induce EAE in rhesus monkeys

J Neuroimmunol. 2007 Jan;182(1-2):135-52. doi: 10.1016/j.jneuroim.2006.10.010. Epub 2006 Nov 28.

Authors

Herbert P M Brok¹, Leonie Boven, Marjan van Meurs, Nicole Kerlero de Rosbo, Liesbeth Celebi-Paul, Yolanda S Kap, Anwar Jagessar, Rogier Q Hintzen, Geoff Keir, Jeffrey Bajramovic, Avraham Ben-Nun, Jan Bauer, Jon D Laman, Sandra Amor, Bert A 't Hart

Affiliation

¹ Department of Immunobiology, Biomedical Primate Research Centre, 2288 GJ Rijswijk, The Netherlands.

PMID: 17126916
DOI: 10.1016/j.jneuroim.2006.10.010

Abstract

Rhesus monkeys immunized with MOG(34-56), a dominant T-cell epitope from myelin/oligodendrocyte glycoprotein, develop an acute neurological disease resembling acute disseminated encephalomyelitis (ADEM) in humans. The typical large demyelinated lesions and mononuclear infiltrates in the monkey brains are caused by MOG(34-56) T-cells. We show that MOG(34-56)-reactive CD4+ and CD8+ T-cells are induced in monkeys immunized with a peptide from the human CMV major capsid protein (UL86; 981-1003), that shares sequence similarity with MOG(34-56). Monkeys sensitized against the viral peptide and subsequently challenged with MOG(34-56) display histological signs of encephalitis, but do not show overt neurological signs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies / blood
Brain / pathology
CD4-Positive T-Lymphocytes / immunology
CD8-Positive T-Lymphocytes / immunology
Capsid Proteins / immunology*
Capsid Proteins / pharmacology
Cell Line
Cell Proliferation / drug effects
Cholesterol / analogs & derivatives*
Cholesterol / immunology
Cross Reactions
Encephalomyelitis, Autoimmune, Experimental / immunology*
Encephalomyelitis, Autoimmune, Experimental / pathology
Encephalomyelitis, Autoimmune, Experimental / physiopathology
Epitopes, T-Lymphocyte / immunology*
Female
Glycoproteins / immunology*
Humans
Lymphocyte Activation
Macaca mulatta / immunology*
Male
Monocytes / pathology
Myelin Proteins
Myelin-Associated Glycoprotein / immunology
Myelin-Associated Glycoprotein / pharmacology
Myelin-Oligodendrocyte Glycoprotein
Peptide Fragments / immunology*
Peptide Fragments / pharmacology
Polyethylene Glycols
Recombinant Proteins / immunology
Spinal Cord / pathology

Substances

Antibodies
Capsid Proteins
Epitopes, T-Lymphocyte
Glycoproteins
MOG protein, human
Myelin Proteins
Myelin-Associated Glycoprotein
Myelin-Oligodendrocyte Glycoprotein
Peptide Fragments
Recombinant Proteins
major capsid protein, Cytomegalovirus
myelin oligodendrocyte glycoprotein (34-56)
polyoxyethanyl 600 cholesteryl sebacate
Polyethylene Glycols
Cholesterol