Mixing two differently labeled substrates in one immobilized metal assay for phosphochemicals assay to improve data quality

Anal Biochem. 2007 Jan 15;360(2):312-4. doi: 10.1016/j.ab.2006.10.024. Epub 2006 Nov 10.
No abstract available

MeSH terms

  • Amino Acid Sequence
  • Carbocyanines / chemistry
  • Checkpoint Kinase 1
  • Fluorescence Resonance Energy Transfer
  • Metals / chemistry*
  • Peptides / chemistry*
  • Phosphates / chemistry*
  • Protein Kinases / chemistry
  • Rhodamines / chemistry
  • Substrate Specificity

Substances

  • 5-carboxytetramethylrhodamine succinimidyl ester
  • Carbocyanines
  • Metals
  • Peptides
  • Phosphates
  • Rhodamines
  • cyanine dye 5
  • Protein Kinases
  • Checkpoint Kinase 1