Efficient generation of 2'-deoxyuridin-5-yl at 5'-(G/C)AA(X)U(X)U-3' (X = Br, I) sequences in duplex DNA under UV-irradiation

Takayoshi Watanabe; Toshikazu Bando; Yan Xu; Hiroshi Sugiyama

doi:10.1093/nass/48.1.19

Efficient generation of 2'-deoxyuridin-5-yl at 5'-(G/C)AA(X)U(X)U-3' (X = Br, I) sequences in duplex DNA under UV-irradiation

Nucleic Acids Symp Ser (Oxf). 2004:(48):19-20. doi: 10.1093/nass/48.1.19.

Authors

Takayoshi Watanabe¹, Toshikazu Bando, Yan Xu, Hiroshi Sugiyama

Affiliation

¹ School of Biomedical Science, Tokyo Medical and Dental University, 2-3-10 Surugadai, Kanda, Chiyoda, Tokyo 101-0062, Japan.

PMID: 17150457
DOI: 10.1093/nass/48.1.19

Abstract

A highly sequence-specific photoreaction of 5'-(G/C)AA(X)U(X)U-3' was found by using 450-base pair DNA fragments containing (X)U under UV-irradiation (X = Br, I). The molecular basis of this high photoreactivity was investigated in detail using synthetic octanucleotides. Based on the experimental data, the mechanism for the efficient photoreaction was proposed.

MeSH terms

Base Sequence
Bromine / chemistry*
DNA / chemical synthesis*
DNA / genetics
DNA / radiation effects*
Iodine / chemistry*
Molecular Sequence Data
Nucleic Acid Heteroduplexes / chemical synthesis*
Nucleic Acid Heteroduplexes / radiation effects
Ultraviolet Rays*
Uridine / chemical synthesis*

Substances

Nucleic Acid Heteroduplexes
DNA
Iodine
Bromine
Uridine