The molecular-thermometer based on B-Z transition of DNA

Ryu Tashiro; Hiroshi Sugiyama

doi:10.1093/nass/48.1.89

The molecular-thermometer based on B-Z transition of DNA

Nucleic Acids Symp Ser (Oxf). 2004:(48):89-90. doi: 10.1093/nass/48.1.89.

Authors

Ryu Tashiro¹, Hiroshi Sugiyama

Affiliation

¹ Division of Biofunctional Molecules, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Surugadai, Kanda, Chiyoda, Tokyo 101-0062, Japan.

PMID: 17150492
DOI: 10.1093/nass/48.1.89

Abstract

A molecular thermometer utilizing the different pi-stacking of B- and Z-DNA was designed. Aminopurine, which is a fluorescent probe for charge-transfer processes, was introduced into B- and Z-DNA and the emission monitored. Z-DNA showed a marked increase in fluorescence compared with B-DNA, with the fluorescence intensity correlating linearly and reproducibly with temperature.

MeSH terms

2-Aminopurine / chemistry
DNA, Z-Form / chemistry*
Nucleic Acid Conformation*
Thermometers*

Substances

DNA, Z-Form
2-Aminopurine