Design of base-discriminating fluorescent (BDF) nucleobase for SNP typing

Yoshio Saito; Keiichiro Kanatani; Yuji Ochi; Akimitsu Okamoto; Isao Saito

doi:10.1093/nass/48.1.243

Design of base-discriminating fluorescent (BDF) nucleobase for SNP typing

Nucleic Acids Symp Ser (Oxf). 2004:(48):243-4. doi: 10.1093/nass/48.1.243.

Authors

Yoshio Saito¹, Keiichiro Kanatani, Yuji Ochi, Akimitsu Okamoto, Isao Saito

Affiliation

¹ NEWCAT Institute, Faculty of Engineering, Nihon University and SORST, Japan Science and Technology Corporation, Tamura, Koriyama 963-8642, Japan.

PMID: 17150569
DOI: 10.1093/nass/48.1.243

Abstract

We have devised novel pyrene-labeled BDF nucleosides, (Py)U, (Py)C, (Py)deazaA, and (Py)A. BDF probes containing these fluorescent nucleosides selectively emit fluorescence only when the base opposite the BDF base is a target base. Oligonucleotides containing these BDF nucleosides act as effective reporter probes for homogeneous SNP typing of DNA samples.

MeSH terms

Fluorescent Dyes / chemistry*
Genes, BRCA2
Humans
Oligonucleotide Probes / chemistry*
Polymorphism, Single Nucleotide / genetics*
Spectrometry, Fluorescence

Substances

Fluorescent Dyes
Oligonucleotide Probes