Promotion of acid-mediated cleavage of oligonucleotide P3'->N5' phosphoroamidates by triplex formation: a novel approach to sequence-specific DNA detection

Nucleic Acids Symp Ser (Oxf). 2005:(49):157-8. doi: 10.1093/nass/49.1.157.

Abstract

The 5'-amino-2',4'-BNA, bearing a locked N-type sugar conformation and a P3'-->N5' phosphoramidate linkage, was found to be rapidly cleaved by acid-mediated hydrolysis when they formed a triplex with the target dsDNA. The fluorophore and quencher moieties were introduced into the 5'-amino-2',4'-BNA-modified oligonucleotide which was used as a novel and innovative DNA detection probe.

MeSH terms

  • DNA / chemistry*
  • DNA Probes / chemistry*
  • Fluorescent Dyes / chemistry
  • Hydrogen-Ion Concentration
  • Hydrolysis
  • Oligonucleotide Probes / chemistry
  • Organophosphorus Compounds / chemistry*
  • Sequence Analysis, DNA*
  • Spectrometry, Fluorescence

Substances

  • DNA Probes
  • Fluorescent Dyes
  • Oligonucleotide Probes
  • Organophosphorus Compounds
  • phosphoramidite
  • triplex DNA
  • DNA