Purpose: To search for an effective fixative solution for retina.
Methods: One group of retinas were fixed in an admixture of formalin, dehydrated alcohol and glacial acetic acid (FFA). The other group of retinas were fixed in 10% neutral formalin.HE and immunohistochemistry were performed. Then compare the difference under the light microscope.
Results: The structure of the retinas fixed in FFA fixative solution was clear. The cells were arranged and compacted. Few exfoliation of the tissue from the glass slide was observed.
Conclusion: FFA fixative solution is superior to 10% neutral formalin for the fixation of retina.