Abstract
The prevalence of CTX-M-producing members of the Enterobacteriaceae is increasing worldwide. A novel, multiplex, real-time TaqMan PCR assay to detect and type bla(CTX-M) genes is described which is an improvement on previously described techniques with respect to reduced assay time, elimination of the need for protracted post-PCR processing and the convenience of a single reaction vessel. Based on beta-lactam antibiogram and MIC data, 478 of 1279 Enterobacteriaceae isolates from clinical blood and urine culture specimens were selected and tested for extended-spectrum beta-lactamase (ESBL) production using phenotypic methods. The new TaqMan assay detected and typed bla(CTX-M) genes in 21 of 28 ESBL-producing isolates.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Ampicillin / pharmacology
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Anti-Bacterial Agents / pharmacology
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Cephalexin / pharmacology
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DNA, Bacterial / chemistry
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DNA, Bacterial / isolation & purification
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Enterobacteriaceae / drug effects
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Enterobacteriaceae / enzymology
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Enterobacteriaceae / genetics*
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Enterobacteriaceae Infections / blood
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Enterobacteriaceae Infections / microbiology*
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Enterobacteriaceae Infections / urine
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Escherichia coli Proteins / genetics
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Genotype
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Humans
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Microbial Sensitivity Tests
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Polymerase Chain Reaction / methods*
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Reproducibility of Results
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Sequence Analysis, DNA
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beta-Lactam Resistance / genetics
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beta-Lactamases / classification
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beta-Lactamases / genetics*
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beta-Lactamases / metabolism
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beta-Lactams / pharmacology
Substances
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Anti-Bacterial Agents
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DNA, Bacterial
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Escherichia coli Proteins
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beta-Lactams
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Ampicillin
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CTX-M-9 protein, E coli
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beta-lactamase CTX-M-15
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beta-Lactamases
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Cephalexin