A Bir1-Sli15 complex connects centromeres to microtubules and is required to sense kinetochore tension

Cell. 2006 Dec 15;127(6):1179-91. doi: 10.1016/j.cell.2006.09.049.

Abstract

Proper connections between centromeres and spindle microtubules are of critical importance in ensuring accurate segregation of the genome during cell division. Using an in vitro approach based on the sequence-specific budding yeast centromere, we identified a complex of the chromosomal passenger proteins Bir1 and Sli15 (Survivin and INCENP) that links centromeres to microtubules. This linkage does not require Ipl1/Aurora B kinase, whose targeting and activation are controlled by Bir1 and Sli15. Ipl1 is the tension-dependent regulator of centromere-microtubule interactions that ensures chromosome biorientation on the spindle. Elimination of the linkage between centromeres and microtubules mediated by Bir1-Sli15 phenocopies mutations that selectively cripple Ipl1 kinase activation. These findings lead us to propose that the Bir1-Sli15-mediated linkage, which bridges centromeres and microtubules and includes the Aurora kinase-activating domain of INCENP family proteins, is the tension sensor that relays the mechanical state of centromere-microtubule attachments into local control of Ipl1 kinase activity.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aurora Kinases
  • Cell Cycle
  • Centromere / metabolism*
  • Chromosomes, Fungal / metabolism
  • DNA, Fungal / metabolism
  • DNA-Binding Proteins / metabolism
  • Fungal Proteins / genetics
  • Fungal Proteins / isolation & purification
  • Fungal Proteins / metabolism*
  • Kinetochores / metabolism*
  • Microtubule-Associated Proteins / metabolism*
  • Microtubules / metabolism*
  • Mitosis
  • Protein Serine-Threonine Kinases / metabolism
  • Protein Structure, Tertiary
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Spindle Apparatus / metabolism*

Substances

  • CBF3b protein, S cerevisiae
  • DNA, Fungal
  • DNA-Binding Proteins
  • Fungal Proteins
  • Microtubule-Associated Proteins
  • Saccharomyces cerevisiae Proteins
  • Sli15 protein, S cerevisiae
  • Aurora Kinases
  • Protein Serine-Threonine Kinases