Abstract
We have cloned a receptor tyrosine kinase cDNA, designated fetal liver kinase 1 (Flk-1), from mouse cell populations enriched for hematopoietic stem and progenitor cells. Sequence analysis of this clone reveals strong homology to the c-Kit subfamily of receptor kinases, and in particular to the Flt gene product. Chromosomal mapping shows that the Flk-1, Kit, and Pdgfra genes are closely linked. Flk-1 mRNA is expressed in primitive and more mature hematopoietic cells as well as in a wide variety of nonhematopoietic tissues.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Chromosome Mapping
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Crosses, Genetic
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DNA / genetics*
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DNA / isolation & purification
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Female
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Fetus
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Gene Expression
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Genetic Linkage*
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Hematopoietic Stem Cells / enzymology*
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Liver / physiology
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Male
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Mice
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Mice, Inbred C57BL
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Molecular Sequence Data
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Multigene Family
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Muridae
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Polymerase Chain Reaction
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Protein-Tyrosine Kinases / genetics*
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Proto-Oncogene Proteins / genetics*
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Proto-Oncogene Proteins c-kit
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Proto-Oncogenes*
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RNA, Messenger / genetics
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Receptors, Cell Surface / genetics*
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Sequence Homology, Nucleic Acid
Substances
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Proto-Oncogene Proteins
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RNA, Messenger
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Receptors, Cell Surface
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DNA
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Protein-Tyrosine Kinases
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Proto-Oncogene Proteins c-kit
Associated data
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GENBANK/S60062
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GENBANK/S60067
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GENBANK/S60069
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GENBANK/S60071
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GENBANK/S60073
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GENBANK/S60076
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GENBANK/S60079
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GENBANK/S60081
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GENBANK/S60382
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GENBANK/X59397