Rapid and highly sensitive pathotyping of avian influenza A H5N1 virus by using real-time reverse transcription-PCR

Bernd Hoffmann; Timm Harder; Elke Starick; Klaus Depner; Ortrud Werner; Martin Beer

doi:10.1128/JCM.01681-06

Rapid and highly sensitive pathotyping of avian influenza A H5N1 virus by using real-time reverse transcription-PCR

J Clin Microbiol. 2007 Feb;45(2):600-3. doi: 10.1128/JCM.01681-06. Epub 2006 Dec 20.

Authors

Bernd Hoffmann¹, Timm Harder, Elke Starick, Klaus Depner, Ortrud Werner, Martin Beer

Affiliation

¹ Institute of Diagnostic Virology, D-17493 Greifswald-Insel Riems, Germany.

Abstract

Rapid typing of the pathogenicity of avian influenza A viruses (AIV) of subtypes H5 and H7 is crucial to initiate adequate protective measures preventing the spread of highly pathogenic AIV (HPAIV). Here, a new real-time reverse transcription-PCR assay which enables sensitive and specific detection and cleavage site analysis of HPAIV H5N1 of the Qinghai lineage is described.

Publication types

Evaluation Study

MeSH terms

Animals
Animals, Wild / virology
Base Sequence
Birds / virology
Chickens / virology
Hemagglutinin Glycoproteins, Influenza Virus / chemistry*
Hemagglutinin Glycoproteins, Influenza Virus / genetics
Hemagglutinin Glycoproteins, Influenza Virus / metabolism
Influenza A Virus, H5N1 Subtype / classification*
Influenza A Virus, H5N1 Subtype / genetics
Influenza A Virus, H5N1 Subtype / pathogenicity*
Influenza in Birds / epidemiology*
Influenza in Birds / prevention & control
Influenza in Birds / virology*
Molecular Sequence Data
Poultry Diseases / virology
Reverse Transcriptase Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Time Factors
Turkeys / virology

Substances

Hemagglutinin Glycoproteins, Influenza Virus
hemagglutinin, avian influenza A virus