Abstract
Dermal fibroblasts from patients with systemic sclerosis (SSc) bound a much greater number of T lymphocytes than did normal dermal fibroblasts. Monoclonal antibodies (MAb) against classes I and II antigens of the major histocompatibility complex (MHC) and their receptors, CD8 and CD4, had no effect on T cell interaction with SSc and normal cells, while MAb against lymphocyte function-associated antigen type 3 (LFA-3) and CD2 both strongly inhibited lymphocyte attachment. MAb against intercellular adhesion molecule type 1 (ICAM-1) and LFA-1 also prevented binding of T lymphocytes, but had a more marked effect on adhesion to SSc fibroblasts than to normal fibroblasts; they also completely abolished the increased binding to fibroblasts treated with interleukin-1 alpha, tumor necrosis factor alpha, and interferon-gamma. No difference was found in the proportion of normal and SSc fibroblasts that expressed MHC classes I and II and LFA-3, but more SSc cells expressed ICAM-1, and at a higher level, than did normal fibroblasts. These results show that cultured SSc cells have elevated binding to T lymphocytes, which possibly results from expansion of a subset of fibroblasts that produces high levels of ICAM-1.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adult
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Aged
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Antibodies, Monoclonal / immunology
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Antigens, Differentiation, T-Lymphocyte / immunology
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Antigens, Differentiation, T-Lymphocyte / metabolism
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Antigens, Surface / genetics*
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Antigens, Surface / immunology
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Antigens, Surface / metabolism
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Antigens, Surface / physiology
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CD2 Antigens
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CD58 Antigens
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Cell Adhesion / drug effects
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Cell Adhesion / physiology
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Cell Adhesion Molecules / immunology
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Cell Adhesion Molecules / metabolism
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Female
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Fibroblasts / cytology
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Fibroblasts / immunology*
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Fibroblasts / pathology
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Humans
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Intercellular Adhesion Molecule-1
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Interferon-gamma / pharmacology
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Interleukin-1 / pharmacology
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Male
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Membrane Glycoproteins / immunology
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Membrane Glycoproteins / metabolism
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Middle Aged
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Receptors, Immunologic / immunology
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Receptors, Immunologic / metabolism
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Scleroderma, Systemic / immunology*
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Scleroderma, Systemic / pathology
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Scleroderma, Systemic / physiopathology
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T-Lymphocytes / metabolism
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T-Lymphocytes / pathology
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T-Lymphocytes / physiology
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Tumor Necrosis Factor-alpha / pharmacology
Substances
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Antibodies, Monoclonal
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Antigens, Differentiation, T-Lymphocyte
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Antigens, Surface
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CD2 Antigens
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CD58 Antigens
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Cell Adhesion Molecules
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Interleukin-1
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Membrane Glycoproteins
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Receptors, Immunologic
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Tumor Necrosis Factor-alpha
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Intercellular Adhesion Molecule-1
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Interferon-gamma