Abstract
Three new withanolide glycosides named daturametelins H-J (1-3), together with two known ones, daturataturin A (4) and 7,27-dihydroxy-1-oxowitha-2,5,24-trienolide (5), were isolated from the MeOH extract of the aerial parts of Datura metel L. (Solanaceae). Their structures were determined mainly by spectroscopic techniques including 2D-NMR (HMBC, HMQC, (1)H,(1)H-COSY, NOESY) and MS experiments. Compounds 1-5 were tested for their antiproliferative activity towards the human colorectal carcinoma (HCT-116) cell line. The nonglycosidic compound 5 exhibited the highest activity of the tested withanolides, with an IC(50) value of 3.2+/-0.2 microM (Table 3).
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents, Phytogenic / chemistry
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Antineoplastic Agents, Phytogenic / isolation & purification*
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Antineoplastic Agents, Phytogenic / pharmacology
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Cell Survival / drug effects
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Datura*
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Drug Screening Assays, Antitumor / methods
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Ergosterol / chemistry
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Ergosterol / isolation & purification*
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Ergosterol / pharmacology
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Glycosides / chemistry
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Glycosides / isolation & purification*
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Glycosides / pharmacology
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HCT116 Cells
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Humans
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Plant Components, Aerial
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification*
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Plant Extracts / pharmacology
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Withania*
Substances
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Antineoplastic Agents, Phytogenic
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Glycosides
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Plant Extracts
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Ergosterol