Abstract
We investigated the effect of KIOM-79, 80% ethanolic extract of a new herbal prescription, on non-obese type 2 diabetic Goto-Kakizaki (GK) rats. The rats were treated orally with KIOM-79 (500 mg/kg body weight) once a day for 13 weeks to examine the long-term effects on hyperglycemia and glomerular histology as well as biochemical and functional abnormalities in kidney. As the results, we found that KIOM-79 reduced hyperglycemia (p<0.01), ameliorated insulin resistance (p<0.001), urinary protein excretion (p<0.01) and creatinine clearance (Ccr) (p<0.001), and inhibited glomerular AGE formation (p<0.001) in diabetic GK rats. We also found that KIOM-79 prevented the glomeruli enlargement, overexpression of type IV collagen (p<0.001), PKC protein (p<0.01), TGF-beta mRNA (p<0.05) and VEGF mRNA (p<0.05). Thus, based on our finding, KIOM-79 could reduce the hyperglycemia, and prevent or retard the development of diabetic nephropathy.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Administration, Oral
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Animals
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Blood Glucose / metabolism
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Collagen Type IV / metabolism
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Creatine / analysis
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Diabetes Mellitus, Experimental / drug therapy*
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Diabetes Mellitus, Experimental / metabolism
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Diabetes Mellitus, Type 2 / drug therapy*
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Diabetes Mellitus, Type 2 / metabolism
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Diabetic Nephropathies / drug therapy*
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Diabetic Nephropathies / metabolism
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Drug Administration Schedule
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Fasting
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Glycation End Products, Advanced / metabolism
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Herbal Medicine*
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Hypoglycemic Agents / administration & dosage
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Hypoglycemic Agents / isolation & purification
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Hypoglycemic Agents / therapeutic use*
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Immunohistochemistry
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Insulin / blood
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Insulin / metabolism
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Insulin Resistance
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Kidney Cortex / drug effects
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Kidney Cortex / metabolism
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Male
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Plant Extracts / pharmacology*
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Protein Kinase C / metabolism
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Proteinuria / prevention & control
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RNA, Messenger / metabolism
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Rats
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Rats, Inbred Strains
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Transforming Growth Factor beta / metabolism
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Vascular Endothelial Growth Factor A / metabolism
Substances
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Blood Glucose
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Collagen Type IV
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Glycation End Products, Advanced
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Hypoglycemic Agents
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Insulin
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KIOM 79
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Plant Extracts
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RNA, Messenger
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Transforming Growth Factor beta
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Vascular Endothelial Growth Factor A
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Protein Kinase C
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Creatine