Human papillomavirus detection in urine samples: an alternative screening method

J Low Genit Tract Dis. 2007 Jan;11(1):5-7. doi: 10.1097/01.lgt.0000230204.65742.e4.

Abstract

Objectives: To investigate the usefulness of human papillomavirus detection in the urine of women with poor gynecologic attention.

Materials and methods: Fifty urine and 50 cervical samples from 50 women were analyzed. Polymerase chain reaction was performed on these 100 samples using consensus primers and a low-density microarray-based method for human papillomavirus typing.

Results: The concordance of the results between both sample groups was 80%. In the urine samples, the sensitivity of polymerase chain reaction for high-grade squamous intraepithelial lesion was 100%, the specificity was 80%, the positive predictive value was 91%, and the negative predictive value was 100%.

Conclusions: Human papillomavirus detection in urine samples may be used as an alternative screening method for women with poor gynecologic attention.

MeSH terms

  • Adult
  • Alphapapillomavirus / isolation & purification*
  • Cervix Uteri / virology
  • DNA, Viral / analysis
  • DNA, Viral / urine
  • Female
  • Humans
  • Mass Screening
  • Middle Aged
  • Oligonucleotide Array Sequence Analysis
  • Papillomavirus Infections / diagnosis*
  • Papillomavirus Infections / urine
  • Polymerase Chain Reaction
  • Sensitivity and Specificity
  • Urine / virology*
  • Uterine Cervical Dysplasia / diagnosis*
  • Uterine Cervical Dysplasia / virology
  • Uterine Cervical Neoplasms / diagnosis*
  • Uterine Cervical Neoplasms / virology

Substances

  • DNA, Viral