Abstract
Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t (1/2) = 43 days at 70 degrees C) in a continuous recycling mode at 70 degrees C produced 49 and 38 g D-tagatose/l from 180 and 90 g D-galactose/l, respectively, within 12 h.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabinose / genetics
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Arabinose / metabolism*
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Cell Culture Techniques / methods
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Escherichia coli / genetics
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Escherichia coli / metabolism*
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Hexoses / biosynthesis*
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Hexoses / genetics
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Isomerases / genetics
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Isomerases / metabolism*
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Protein Engineering / methods*
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Recombinant Proteins / metabolism
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Sweetening Agents / metabolism*
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Temperature
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Thermotoga neapolitana / enzymology*
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Thermotoga neapolitana / genetics
Substances
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Hexoses
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Recombinant Proteins
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Sweetening Agents
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Arabinose
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Isomerases
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tagatose